Journal: Scientific Reports

Article Title: Hepatocyte growth factor secreted by bone marrow stem cell reduce ER stress and improves repair in alveolar epithelial II cells

doi: 10.1038/srep41901

Figure Lengend Snippet: The cells stained for E cadherin performed by immuno-fluroscence ( a ), the cells were also stained for surfactant protein A, positive cells stain pink by alkaline phosphatase staining ( b ). Cells also stained for Surfactant protein C (SP-c) as shown with positive immunoflurosence ( c ). Immunostaining for the ER stress marker GRP78 was performed on the paraffin embedded IPF lung tissue. A distinct staining of the epithelial lining for Grp 78 was seen in area around thickened interstitium. Single mononuclear cell lining the airspaces showed strong staining, based on their location and shape they are likely the alveolar epithelial cells ( d ). Grp78 positive cells (red) are also positive for SPC (green) thus confirming that AEC are under ER stress in IPF patients ( e ). (Image processed by Imaris for volume rendering for clear view) (Scale bar ( a–c ) (50 μm), ( d ) (500 μm), ( e ) (10 μm).

Article Snippet: Peripheral lung tissue was cut into small pieces of approximately 1 mm 3 , and placed into pre-wetted 25 cm 2 cell culture flasks (Falcon, Corning Incorporated NY, USA) by 1 ml Epithelial growth medium (CELLnTEC, Bern, Switzerland) no additional coating was applied.

Techniques: Staining, Immunostaining, Marker

Journal: Scientific Reports

Article Title: Hepatocyte growth factor secreted by bone marrow stem cell reduce ER stress and improves repair in alveolar epithelial II cells

doi: 10.1038/srep41901

Figure Lengend Snippet: Relative mRNA expression of the ER stress markers measured in primary alveolar epithelial cells isolated from the IPF patients by RT PCR; relative gene expression levels of GRP78 ( a ) CHOP ( b ) and XBP-1 ( c ) normalized to primary alveolar epithelial cells obtained from healthy lungs are demonstrated. After treatment with BMSC-cm the gene expression of GRP78 and CHOP was significantly reduced, however there was no significant change in spliced XBP-1. Moreover, there was no significant increase in the mRNA expression of any ER stress marker after neutralizing HGF in the BMSC-cm. Relative gene expression is represented as ΔΔCT and expressed as mean ± SEM. Experiments were performed in triplicates (n = 3).

Article Snippet: Peripheral lung tissue was cut into small pieces of approximately 1 mm 3 , and placed into pre-wetted 25 cm 2 cell culture flasks (Falcon, Corning Incorporated NY, USA) by 1 ml Epithelial growth medium (CELLnTEC, Bern, Switzerland) no additional coating was applied.

Techniques: Expressing, Isolation, Reverse Transcription Polymerase Chain Reaction, Gene Expression, Marker

Journal: Scientific Reports

Article Title: Hepatocyte growth factor secreted by bone marrow stem cell reduce ER stress and improves repair in alveolar epithelial II cells

doi: 10.1038/srep41901

Figure Lengend Snippet: Additionally these cells lost cytokeratin ( d,j ) and E cadherin ( f , l ) expression but expressed vimentin ( e,k ). Treatment with BMSC-cm helped restore the morphology of the ER stressed A549 cells, back to that of the epithelial cells, the expression of vimentin was lost ( h,n ) cytokeratin ( g,m ) and E cadherin ( i,o ) expressions were restored. (Scale bar 20 μm for all figures). Experiments were performed in triplicates (n = 3).

Article Snippet: Peripheral lung tissue was cut into small pieces of approximately 1 mm 3 , and placed into pre-wetted 25 cm 2 cell culture flasks (Falcon, Corning Incorporated NY, USA) by 1 ml Epithelial growth medium (CELLnTEC, Bern, Switzerland) no additional coating was applied.

Techniques: Expressing

Journal: Scientific Reports

Article Title: Hepatocyte growth factor secreted by bone marrow stem cell reduce ER stress and improves repair in alveolar epithelial II cells

doi: 10.1038/srep41901

Figure Lengend Snippet: Data is shown as % of alveolar epithelial wound healing and values are expressed as mean ± SEM. Experiments were performed in triplicates (n = 3).

Article Snippet: Peripheral lung tissue was cut into small pieces of approximately 1 mm 3 , and placed into pre-wetted 25 cm 2 cell culture flasks (Falcon, Corning Incorporated NY, USA) by 1 ml Epithelial growth medium (CELLnTEC, Bern, Switzerland) no additional coating was applied.

Techniques:

Journal: Scientific Reports

Article Title: Hepatocyte growth factor secreted by bone marrow stem cell reduce ER stress and improves repair in alveolar epithelial II cells

doi: 10.1038/srep41901

Figure Lengend Snippet: Data is represented as % of alveolar epithelial wound healing and values are expressed as Mean ± SEM. Experiments were performed in triplicates (n = 3).

Article Snippet: Peripheral lung tissue was cut into small pieces of approximately 1 mm 3 , and placed into pre-wetted 25 cm 2 cell culture flasks (Falcon, Corning Incorporated NY, USA) by 1 ml Epithelial growth medium (CELLnTEC, Bern, Switzerland) no additional coating was applied.

Techniques: